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Cloning and Expression
Enzymes
Acc65I
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Acc65I
Acc65I from New England Biolabs
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Citations:
(12)
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New England Biolabs for
Acc65I
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Acc65I
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0599S
Concentration
10 u/µL
Quantity
2000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
G/GTACC
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (12)
(1)
CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuits
Nucleic Acids Research
February 22, 2023
Lifang Yu, Mario Andrea Marchisio
terminator), into a cut-open pRSII405 backbone via Gibson method. pRSII405 was digested with
Acc65I
(
NEB
-
R0599S
) and
SacI-HF
(
NEB
-
R3156S
). TUs containing As/LbCas12a or LwaCas13a repeat along the 5′ UTR or MbCas12a
(2)
Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol
Scientific Reports
December 16, 2022
Tian Zhou, Zhiying Liang, Mario Andrea Marchisio
integrative backbone was cut-open (5 µg in a 30 µl solution) with the restriction endonucleases
Acc65I
(
NEB
-
R0599S
) and
SacI
(
NEB
-
R0156S
) to remove the MCS. At the end of the digestion, the two enzymes were heat-inactivated
(3)
A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae
Frontiers in Bioengineering and Biotechnology
June 2, 2022
Yadan Zhang, Huanhuan Ge, Mario Andrea Marchisio
purified as above. The pRSII shuttle vector was digested with
SacI-HF
(
NEB
-
R3156S
) and
Acc65I
(
NEB
-
R0599S
) for 1 h at 37°C to generate a cut-open backbone that was then incubated for 20 min at 65°C to inactivate
(4)
Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress
Molecular cell
April 21, 2022
Stoneley M, Harvey RF, Mulroney TE, Mordue R, Jukes-Jones R, Cain K, Lilley KS, Sawarkar R, Willis AE.
,
New England Biolabs
,Cat. #
R3136
XhoI
,
New England Biolabs
,Cat. #R0146
Acc65I
,
New England Biolabs
,Cat. #
R0599
ApaI
,
New England Biolabs
,Cat. #
R0114
XbaI
,
New England Biolabs
,Cat. #
R0145S
HindIII
,
New England Biolabs
,Cat.
(5)
Analysis of Drosophila Atg8 proteins reveals multiple lipidation-independent roles
Autophagy
September 2, 2021
András Jipa, Viktor Vedelek, Zsolt Merényi, Adél Ürmösi, Szabolcs Takáts, Attila L. Kovács, Gábor V. Horváth, Rita Sinka, Gábor Juhász
3ʹ-UTR region of Atg8b was amplified using GATATAGCGGCCGCGGAGGTGGCATGGATATGAACTA CCAGTACAAGAAGG and CTCGAGGTACCAGCCGATCAGTTTGCACGCTTC primers and cloned it into the above vector as a
NotI
(
NEB
, R3189)
Acc65I
(
NEB
,
R0599S
) fragment. To generate the C-terminal FLAG-containing Atg8b con struct lacking the glycine at position
(6)
Novel S. cerevisiae Hybrid Synthetic Promoters Based on Foreign Core Promoter Sequences
International Journal of Molecular Sciences
May 27, 2021
Xiaofan Feng, Mario Andrea Marchisio
the pRSII406 vector was cut-open with
Acc65I
(
NEB
- Int. J. Mol. Sci. 2021, 22, 5704 13 of 15
R0599S
) and
SacI
(
NEB
-
R0156S
). Touchdown
PCR
[51] was used to extract DNA sequences from their original plasmids.
(7)
The ubiquitin ligase RFWD3 is required for translesion DNA synthesis
Molecular Cell
February 4, 2021
Irene Gallina et al.
,
New England BioLabs
,Cat#
M0262S
Exonuclease I,
New England BioLabs
,Cat#
M0293S
Acc65I
restriction enzyme,
New England BioLabs
,Cat#
R0599L
HincII restriction enzyme,
New England BioLabs
,Cat#
R0103S
SapI
restriction enzyme,
New England BioLabs
,Cat#
R0596S
"Proteinase
(8)
Distinct subnetworks of the thalamic reticular nucleus
Nature
July 30, 2020
Yinqing Li et al.
to remove the Cre from pAAV60231, pAAV60231 was digested by
Acc65I
and
EcoRI
(
New England Biolabs
, #
R0599
and #
R3101
) and the plasmid backbone was agarose gel purified. EGFP-KASH was
PCR
amplified from pAAV60231
(9)
An intestinal zinc sensor regulates food intake and developmental growth
Nature
April 1, 2020
Redhai S, Pilgrim C, Gaspar P, Giesen LV, Lopes T, Riabinina O, Grenier T, Milona A, Chanana B, Swadling JB,
et al
.
R (see below). The
PCR
products were cloned into pTV Cherry using
NotI
and Acc651 (
NEB
#
R0189
and #
R0599
, respectively) or
AscI
and
SpeI
(
NEB
#
R0558
and #R3133, respectively) restriction enzymes. The completed
(10)
A Bicistronic Plasmid Encoding Brain-Derived Neurotrophic Factor and Urokinase Plasminogen Activator Stimulates Peripheral Nerve Regeneration After Injury
Journal of Pharmacology and Experimental Therapeutics
March 1, 2020
Maxim Karagyaur, Alexandra Rostovtseva, Ekaterina Semina, Polina Klimovich, Vadim Balabanyan, Pavel Makarevich, Vladimir Popov, Dmitry Stambolsky, Vsevolod Tkachuk
(
New England Biolabs
, #
R0104S
) restriction sites (Kozak, 1984). huPA cDNA (cloned using
Acc65I
(
New England Biolabs
, #
R0599S
) and
EagI
(
New England Biolabs
, #
R0505S
) restriction sites) was separated from hBDNF by HCV IRES (Lukavsky, 2009),
(11)
FGF20-expressing, Wnt-Responsive Olfactory Epithelial Progenitors Regulate Growth of Underlying Turbinates to Optimize Epithelial Surface Area
Developmental Cell
September 1, 2018
Lu M. Yang, Sung-Ho Huh, David M. Ornitz
make antisense probe, the plasmid was linearized with restriction enzyme
Acc65I
(
New England Biolabs
,
R0599S
) and transcribed with
T7 RNA polymerase
(
New England Biolabs
,
M0251S
) according to manufacturer’s instructions,
(12)
A HIF-LIMD1 negative feedback mechanism mitigates the pro-tumorigenic effects of hypoxia
EMBO molecular medicine
August 1, 2018
Foxler DE, Bridge KS, Foster JG, Grevitt P, Curry S, Shah KM, Davidson KM, Nagano A, Gadaleta E, Rhys HI,
et al
.
annealed and ligated into psiRNA‐DUO plasmid (
Invivogen
#
ksirna4‐gz3
) with
Acc65I
and
HindIII
(
NEB
#
R0599S
and #
R0104S
). The
knockdown
‐rescue
shRNA
lentiviral system was a kind gift from Greg Longmore (Feng
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Acc65I from New England Biolabs
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